Clive Wasserfall, MS

WassResizeResearch Interests

Our laboratory focus is on the immunopathogenesis of type 1 diabetes. Aspects of this work include refining prediction of disease onset/progression through the use of genetic markers and autoantibody assessment. The mechanism of disease is explored on the cellular and molecular level in order to better understand the pathogenesis of this disease and thereby lead to the goal of our program which is to prevent type 1 diabetes in those at risk. The induction of immune tolerance to the insulin secreting endocrine pancreas is the current strategy being pursued for both primary intervention of disease and to improve islet transplantation without the need for immunosuppression in those individuals that already have type 1 diabetes.



Featured Publications:

1. Villa NY, Wasserfall CH, Meacham AM, Wise E, Chan W, Wingard JR, McFadden G, Cogle CR. Myxoma virus suppresses proliferation of activated T lymphocytes yet permits oncolytic virus transfer to cancer cells. Blood. 2015 Jun 11;125(24):3778-88. doi: 10.1182/blood-2014-07-587329. Epub 2015 Apr 22. PubMed PMID: 25904246.

2. Lewis JS, Dolgova NV, Zhang Y, Xia CQ, Wasserfall CH, Atkinson MA, Clare-Salzler MJ, Keselowsky BG. A combination dual-sized microparticle system modulates dendritic cells and prevents type 1 diabetes in prediabetic NOD mice. Clin Immunol. 2015 Apr 2. pii: S1521-6616(15)00126-6. doi:10.1016/j.clim.2015.03.023. [Epub ahead of print] PubMed PMID: 25842187.

3. Bian X, Wiktor P, Kahn P, Brunner A, Khela A, Karthikeyan K, Barker K, Yu X, Magee M, Wasserfall CH, Gibson D, Rooney ME, Qiu J, LaBaer J. Antiviral antibody profiling by high-density protein arrays. Proteomics. 2015 Jun;15(12):2136-45. doi: 10.1002/pmic.201400612. Epub 2015 May 15. PubMed PMID: 25758251.

4. Haller MJ, Gitelman SE, Gottlieb PA, Michels AW, Rosenthal SM, Shuster JJ, Zou B, Brusko TM, Hulme MA, Wasserfall CH, Mathews CE, Atkinson MA, Schatz DA. Anti-thymocyte globulin/G-CSF treatment preserves β cell function in patients with established type 1 diabetes. J Clin Invest. 2015 Jan;125(1):448-55. doi:
10.1172/JCI78492. Epub 2014 Dec 15. PubMed PMID: 25500887; PubMed Central PMCID: PMC4382237.

5. Kweh FA, Miller JL, Sulsona CR, Wasserfall C, Atkinson M, Shuster JJ, Goldstone AP, Driscoll DJ. Hyperghrelinemia in Prader-Willi syndrome begins in early infancy long before the onset of hyperphagia. Am J Med Genet A. 2015 Jan;167A(1):69-79. doi: 10.1002/ajmg.a.36810. Epub 2014 Oct 29. PubMed PMID: 25355237; PubMed Central PMCID: PMC4391201.

6: Kleffel S, Vergani A, Tezza S, Ben Nasr M, Niewczas MA, Wong S, Bassi R, D’Addio F, Schatton T, Abdi R, Atkinson M, Sayegh MH, Wen L, Wasserfall CH, O’Connor KC, Fiorina P. Interleukin-10+ regulatory B cells arise within antigen-experienced CD40+ B cells to maintain tolerance to islet autoantigens. Diabetes. 2015 Jan;64(1):158-71. doi: 10.2337/db13-1639. Epub 2014 Sep 3. PubMed PMID: 25187361; PubMed Central PMCID: PMC4274804.

7. Cara-Fuentes G, Wasserfall CH, Wang H, Johnson RJ, Garin EH. Minimal change disease: a dysregulation of the podocyte CD80-CTLA-4 axis? Pediatr Nephrol. 2014
Dec;29(12):2333-40. doi: 10.1007/s00467-014-2874-8. Epub 2014 Jul 14. PubMed PMID: 25017621; PubMed Central PMCID: PMC4213236.

8: Radichev IA, Maneva-Radicheva LV, Amatya C, Parker C, Ellefson J, Wasserfall C, Atkinson M, Burn P, Savinov AY. Nardilysin-dependent proteolysis of cell-associated VTCN1 (B7-H4) marks type 1 diabetes development. Diabetes. 2014 Oct;63(10):3470-82. doi: 10.2337/db14-0213. Epub 2014 May 21. PubMed PMID: 24848066; PubMed Central PMCID: PMC4171653.

9: Lewis JS, Roche C, Zhang Y, Brusko TM, Wasserfall CH, Atkinson M, Clare-Salzler MJ, Keselowsky BG. Combinatorial delivery of immunosuppressive factors to dendritic cells using dual-sized microspheres. J Mater Chem B Mater Biol Med. 2014 May 7;2(17):2562-2574. PubMed PMID: 24778809; PubMed Central PMCID: PMC4000038.

10. Robert S, Gysemans C, Takiishi T, Korf H, Spagnuolo I, Sebastiani G, Van Huynegem K, Steidler L, Caluwaerts S, Demetter P, Wasserfall CH, Atkinson MA, Dotta F, Rottiers P, Van Belle TL, Mathieu C. Oral delivery of glutamic acid decarboxylase (GAD)-65 and IL10 by Lactococcus lactis reverses diabetes in recent-onset NOD mice. Diabetes. 2014 Aug;63(8):2876-87. doi: 10.2337/db13-1236. Epub 2014 Mar 27. PubMed PMID: 24677716.

11. Pugliese A, Yang M, Kusmarteva I, Heiple T, Vendrame F, Wasserfall C, Rowe P, Moraski JM, Ball S, Jebson L, Schatz DA, Gianani R, Burke GW, Nierras C, Staeva
T, Kaddis JS, Campbell-Thompson M, Atkinson MA. The Juvenile Diabetes Research Foundation Network for Pancreatic Organ Donors with Diabetes (nPOD) Program:
goals, operational model and emerging findings. Pediatr Diabetes. 2014 Feb;15(1):1-9. doi: 10.1111/pedi.12097. Epub 2013 Dec 10. Review. PubMed PMID:
24325575; PubMed Central PMCID: PMC4282794.

12: Garrigan E, Belkin NS, Alexander JJ, Han Z, Seydel F, Carter J, Atkinson M, Wasserfall C, Clare-Salzler MJ, Amick MA, Litherland SA. Persistent STAT5 phosphorylation and epigenetic dysregulation of GM-CSF and PGS2/COX2 expression in Type 1 diabetic human monocytes. PLoS One. 2013 Oct 18;8(10):e76919. doi: 10.1371/journal.pone.0076919. eCollection 2013. PubMed PMID: 24204704; PubMed Central PMCID: PMC3799903.

13. Miersch S, Bian X, Wallstrom G, Sibani S, Logvinenko T, Wasserfall CH, Schatz D, Atkinson M, Qiu J, LaBaer J. Serological autoantibody profiling of type 1 diabetes by protein arrays. J Proteomics. 2013 Dec 6;94:486-96. doi: 10.1016/j.jprot.2013.10.018. Epub 2013 Oct 20. PubMed PMID: 24148850.

14. Rowe P, Wasserfall C, Croker B, Campbell-Thompson M, Pugliese A, Atkinson M, Schatz D. Increased complement activation in human type 1 diabetes pancreata.
Diabetes Care. 2013 Nov;36(11):3815-7. doi: 10.2337/dc13-0203. Epub 2013 Sep 16. PubMed PMID: 24041678; PubMed Central PMCID: PMC3816899.

15. Myhr CB, Hulme MA, Wasserfall CH, Hong PJ, Lakshmi PS, Schatz DA, Haller MJ, Brusko TM, Atkinson MA. The autoimmune disease-associated SNP rs917997 of IL18RAP controls IFNγ production by PBMC. J Autoimmun. 2013 Aug;44:8-12. doi: 10.1016/j.jaut.2013.06.001. Epub 2013 Jul 24. PubMed PMID: 23891168; PubMed
Central PMCID: PMC3775347.

16. Villalta SA, Lang J, Kubeck S, Kabre B, Szot GL, Calderon B, Wasserfall C, Atkinson MA, Brekken RA, Pullen N, Arch RH, Bluestone JA. Inhibition of VEGFR-2
reverses type 1 diabetes in NOD mice by abrogating insulitis and restoring islet function. Diabetes. 2013 Aug;62(8):2870-8. doi: 10.2337/db12-1619. Epub 2013 Jul
8. PubMed PMID: 23835340; PubMed Central PMCID: PMC3717875.

17. Ishimoto T, Cara-Fuentes G, Wang H, Shimada M, Wasserfall CH, Winter WE, Rivard CJ, Araya CE, Saleem MA, Mathieson PW, Johnson RJ, Garin EH. Serum from
minimal change patients in relapse increases CD80 expression in cultured podocytes. Pediatr Nephrol. 2013 Sep;28(9):1803-12. doi: 10.1007/s00467-013-2498-4. Epub 2013 May 21. PubMed PMID: 23689904; PubMed Central PMCID: PMC3723775.

18. Haller MJ, Wasserfall CH, Hulme MA, Cintron M, Brusko TM, McGrail KM, Wingard JR, Theriaque DW, Shuster JJ, Ferguson RJ, Kozuch M, Clare-Salzler M, Atkinson
MA, Schatz DA. Autologous umbilical cord blood infusion followed by oral docosahexaenoic acid and vitamin D supplementation for C-peptide preservation in children with Type 1 diabetes. Biol Blood Marrow Transplant. 2013 Jul;19(7):1126-9. doi: 10.1016/j.bbmt.2013.04.011. Epub 2013 Apr 20. PubMed PMID: 23611977; PubMed Central PMCID: PMC3852705.

19. Lantow M, Sivakumar R, Zeumer L, Wasserfall C, Zheng YY, Atkinson MA, Morel L. The granulocyte colony stimulating factor pathway regulates autoantibody production in a murine induced model of systemic lupus erythematosus. Arthritis Res Ther. 2013 Apr 8;15(2):R49. doi: 10.1186/ar4208. PubMed PMID: 23566364; PubMed Central PMCID: PMC3672747.

20. Sarikonda G, Sachithanantham S, Manenkova Y, Kupfer T, Posgai A, Wasserfall C, Bernstein P, Straub L, Pagni PP, Schneider D, Calvo TR, Coulombe M, Herold K, Gill RG, Atkinson M, Nepom G, Ehlers M, Staeva T, Garren H, Steinman L, Chan AC, von Herrath M. Transient B-cell depletion with anti-CD20 in combination with proinsulin DNA vaccine or oral insulin: immunologic effects and efficacy in NOD mice. PLoS One. 2013;8(2):e54712. doi: 10.1371/journal.pone.0054712. Epub 2013 Feb 6. PubMed PMID: 23405091; PubMed Central PMCID: PMC3566105.